Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination ofTaqPolymerase
نویسندگان
چکیده
منابع مشابه
Real-time multiplex PCR assays.
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
متن کاملComparison of 16S rDNA-PCR Amplification and Culture of Cerebrospinal Fluid for Diagnosis of Bacterial Meningitis
OBJECTIVE Early and accurate diagnosis of bacterial meningitis is of critical concern. Optimum and rapid laboratory facilities are not routinely available for detecting the etiologic agents of meningitis. The objective of this study was to compare polymerase chain reaction (PCR) assay with culture for detection of bacteria in central nervous system (CNS) samples from patients suspected to have ...
متن کاملWhat is broad-range 16S rDNA PCR?
Patel A, et al. Arch Dis Child Educ Pract Ed 2017;0:1–4. doi:10.1136/archdischild-2016-312049 INTRODUCTION PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s. Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of particular bacterial species. The technique uses specific primers (short strands of nuclei...
متن کاملEvaluation of Real-Time 16S rDNA PCR and Pyrosequencing for Routine Identification of Bacteria in Joint Fluid and Tissue Specimens
16S rDNA PCR and sequencing are powerful tools for bacterial detection and identification, although their routine use is not currently widespread in the field of clinical microbiology. The availability of pyrosequencing now makes 16S rDNA assays more accessible to routine diagnostic laboratories, but this approach has had limited evaluation in general diagnostic practice. In this study we evalu...
متن کاملApplication of 16S rDNA based seminested PCR for diagnosis of acute bacterial meningitis.
BACKGROUND & OBJECTIVES The diagnosis of bacterial meningitis remains a challenge to the clinician because of its rapid lethal course lacking the consistency to particular clinical signs and symptoms. Moreover, in many clinical settings use of rampant and short course antibiotic therapy prior to lumbar puncture reduces the chance of isolation of bacteria in CSF culture making the diagnosis diff...
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ژورنال
عنوان ژورنال: Transfusion Medicine and Hemotherapy
سال: 2010
ISSN: 1660-3818,1660-3796
DOI: 10.1159/000265571